WebTo evaluate the time-dependent uptake of OVA/CpG and OVA-CpG NPs in DCs, DCs cultured in a 24-well plate were incubated with Cy5-OVA/FAM-CpG and Cy5-OVA-FAM-CpG NPs for different time intervals until 24 h. In each group, 10 μg soluble OVA or an equivalent dose of OVA NPs loaded with or without 1 μg CpG was used. Then, cells … WebThe dual-functional delivery system PEI/CaCO 3 (polyethylenimine coated CaCO 3 ), could not only act as a vaccine carrier that absorbed antigen ovalbumin (OVA) and adjuvant unmethylated cytosine-phosphate-guanine (CpG) with high efficiency, but also work as an underlying adjuvant to activate bone marrow-derived dendritic cells (BMDCs).
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WebMar 24, 2024 · The ability of the CpG/ISCOMATRIX combination to induce antitumor responses when administered with OVA following administration to mice of a highly metastatic OVA-secreting tumor cell line (B16-OVA melanoma) was demonstrated and may prove to be a valuable tool in the development of novel or improved vaccines. WebJan 5, 2024 · The doses of OVA and CpG were 100 μg per mouse and 25 μg per mouse, respectively. For analyses of the biocompatibility, main organs (heart, liver, lung and kidney) were isolated from vaccinated mice on day 21, H&E staining of these tissue sections was performed and the digital microscope images were recorded. highest island
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WebJan 1, 2024 · The CpG–OVA conjugate was purified from unconjugated CpG using preparative SEC. The conjugation ratio of CpG to OVA was determined by measuring the formation of the bis-aryl hydrazone bond by the change in absorption at 354 nm. The 5′ and 3′ CpG–OVA conjugates were calculated to have the same level of conjugation of 2.8 ± … WebA .cpg file represents the default drawing file format. Updated: July 25, 2024. The default software associated to open cpg file: Manga Studio . Company or developer: Smith … WebJun 29, 2024 · CpG oligonucleotides (ODN), immunomodulatory synthetic oligonucleotides designed to stimulate TLR9 (Toll-like receptor 9), are expressed on human plasmacytoid dendritic cells and B cells. CpG ODNs trigger an innate immune response characterized by the production of Th1 and proinflammatory cytokines [ 12 ]. highest iso camera