Hygromycin phosphotransferase hpt gene
Web1 jan. 2013 · To obtain HPT protein (Hygromycin B Phosphotransferase), a kind of plant selective maker gene product expressed from E. coli and to prepare the polyclonal … Web21 jan. 2024 · The CRISPR/Cas9 technique for rice genome engineering is gaining momentum and requires a precise gene delivery system. For rice and other crop plants, …
Hygromycin phosphotransferase hpt gene
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WebThe hygromycin phosphotransferase gene (hpt) from E. coli under the control of the SV40 early promoter was used as a dominant selectable marker for transformation of … Web1) hygromycin phosphotransferase (hpt) 潮霉素磷酸转移酶基因 (hpt) 2) hygB 潮霉素磷酸转移酶基因 1. We transformed the nematode-trapping fungus Arthrobotrys vermicola …
WebThe aim of this work is to evaluate the use of the hygromycin phosphotransferase (hpt) gene as the selectable marker for generation of stable oil palm transformation via … WebA total of 3.46 x 10(8) protoplasts isolated from 17 independent transgenic Arabidopsis lines harbouring the defective chimeric hpt gene were transformed via direct gene …
WebDownload scientific diagram The plasmid I used in this study with hygromycin phosphotransferase gene (hygromycin resistance, Hyg (R), near the left T-DNA border). WebA transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which contains a bacterial hygromycin B phosphotransferase gene linked to Cochliobolus heterostrophus regulatory sequences (B. G. Turgeon, R. C. Garber, and O. C. Yoder, …
Web1 aug. 2004 · Hygromycin B phosphotransferase (HPT) is a widely used selectable marker protein of transgenic plant. Detection of its activity is critical to studies on the …
WebObjective This article studied on the food safety of HPT protein (Hygromycin B Phosphotransferase), a kind of plant selectable marker gene expression product, which … hope initiative rhode islandWeb24 sep. 2011 · Of three major selectable genes, the bialaphos resistance (bar) gene and the hygromycin phosphotransferase (hpt) allowed effective selection of transformed cells … long sconce lightsWebA transformation system for the ringworm-producing dermatophyte Trichophyton mentagrophytes has been developed. The system employs the plasmid pHIS, which … hope initiative new castle indianaWebselect transformants expressing the APT gene, which encodes an aminoglycoside phosphotransferase. Hygromycin B (HygB, Wako Pure Chemical Industries, Osaka, Japan) was added to YPD to a final concentration of 600mg/ml to select for transformants carrying the HPT gene, encoding HygB phosphotransferase. longs covid testing waimeaWebEmbryogenic maize (Zea mays L.) callus cultures were transformed by microprojectile bombardment with a chimeric hygromycin phosphotransferase (HPT) gene and three … hope initiative rochester nyWebDirect gene transfer has proved to be an efficient transformation method for arabidopsis thaliana, a member of the Brassicaceae.Transgenic Arabidopsis plants resistant to … long scraggy ranch coloradoWeb25 sep. 2015 · To date, however, only the hygromycin phosphotransferase gene (hpt) has been available for use as a marker for Agrobacterium-mediated transformation in M. … hope initiative mayfield ky