Web本产品稳定性好。. 本试剂盒中的CellTiter-Lumi™ Steady发光法检测试剂稳定性好，反复冻融5次对检测效果基本无影响，反复冻融10次检测效果下降不超过10%。. 本产品4℃保存3天对检测效果无显著影响，4℃保存7天检测效果下降不超过10%。. 室温保存1天，仍可保留80% ... WebSteady-Glo® Luciferase Assay System is designed for this purpose—providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half …
Steady-Glo® Luciferase Assay System Protocol - Promega
WebSteady-Glo® Substrate: Use as supplied. Store at –20°C, where it is stable for up to 6 months. The substrate also may be stored at 4°C for up to 1 month. Steady-Glo® Buffer: Use as supplied. Store below 25°C. Steady-Glo® Reagent: Transfer the contents of one bottle of Steady-Glo® Buffer to one bottle of Steady-Glo® Substrate. Mix WebApr 12, 2024 · The fluorescence was measured using a fluorometer (380–400 nm Ex/505 nm Em). For the luciferase activity, Steady-Glo® Luciferase assay reagent (Promega, WI, USA) prepared according to manufacturer's instructions, was added directly into the assay plates after the cell viability assay. The luciferase activity was measured using a … chris adamson football
Promega Steady-Glo
WebMay 6, 2024 · At each time point, 50 μl of room temperature Steady-Glo luciferin substrate (Promega) was thoroughly mixed with 50 μl of suspended cells. Bioluminescence was read using the Synergy H1 plate reader (Biotek). A CD19-CD3 BiAb-positive control was produced using MAB1794 (EMD Millipore) and included for comparison. ... WebIdeal for use in high-density (384- and 1536-well) microplates. Brighter, Longer-Lasting Signal. Optimized for batch and continuous-process handling, the extended bright light output allows high sensitivity, especially for extended incubations. Reduced Unwanted Effects from Sample Components. WebGlo Lysis Buffer, 1X Part Numbers: E2661 Promotes Rapid Lysis of Cultured Mammalian Cells No need for cell scraping or freeze-thaw cycles Cells lyse within 5 minutes Compatible with Bright-Glo™, Steady-Glo®, ONE-Glo™ … genshin 5 star bow characters