Thp 1 cell lines
WebThe THP-1 cell line is a human monocytic leukaemia cell line which was established in 1980 by Tsuchiya et al. . It was derived from the blood of a patient with acute monocytic … WebJan 5, 2004 · In contrast, the EBNA1 gene was not detected in the THP-1 reference lines, Ramos B cells (an EBV-negative, Burkitt's lymphoma line, Klein et al., 1975), or other human non-B cell lines that we examined including Hut78, Jurkat, HL-60, U937, and K562 cells. B-THP-1 cells are genotypically identical to Raji cells.
Thp 1 cell lines
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WebCatalog number: K1672B. Tumor necrosis factor-alpha (TNFα) cytokine cell signaling is a critical pathway involved in immune and inflammatory responses. In addition, TNFα has been shown to inhibit growth and induce differentiation in tumor and non-tumor cells. The CellSensor™ TNFα-bla THP-1 cell line is a suspension cell line derived from ... THP-1 is a human monocytic cell line derived from an acute monocytic leukemia patient. It is used to test leukemia cell lines in immunocytochemical analysis of protein-protein interactions, and immunohistochemistry.
WebArticle Title: Transcriptome Analysis of Diffuse Large B-Cell Lymphoma Cells Inducibly Expressing MyD88 L265P Mutation Identifies Upregulated CD44, LGALS3, NFKBIZ, and … WebMay 11, 2015 · Understanding how immune cells such as macrophages interact with cancer cells is of increasing interest, as cancer treatments move towards combining both targeted- and immuno- therapies in new treatment regimes. This protocol is using THP-1 cells, a human leukemia monocytic cell line that can be differentiated into macrophages.
Webconcentration of diclofenac sodium and selonsertib, first the THP-1 cells were cultured in 96-well culture plates at 5x103 cells per well. Because the THP-1 cells are a suspended cell line, the drugs were administered on the same day. The concentrations determined for diclofenac sodium and selonsertib were between 1.56-400 μM/mL. WebThe monocytic cell line THP-1 can be stimulated to produce IFN-β when challenged with purified dsDNA oligomers > 80 bp, plasmids, bacterial DNA and human DNA. We have also …
WebFeb 28, 2024 · Models based on cell cultures have become a useful tool in modern scientific research. Since primary cell lines are difficult to obtain and handle, neoplasm-derived lines like PC12 and THP-1 offer a cheap and flexible solution for neurobiological studies but require prior differentiation to serve as a neuronal or microglia model. PC12 cells …
WebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM … riseup northWebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a temperature of 37 o C in a humidified incubator with … rise up nutrition new port richeyWebAll lanes : Anti-SHP1 antibody [Y476] at 1/1000 dilution Lane 1 : Wild-type THP-1 cell lysate Lane 2 : PTPN6 knockout THP-1 cell lysate Lane 3 : Jurkat cell lysate Lane 4 : K562 cell lysate Lysates/proteins at 20 µg per lane. Performed under reducing conditions. Predicted band size: 68 kDa Observed band size: 70 kDa why is the actual band size different from … rise up nutrition north smithfield riWebNov 1, 2014 · The THP-1 cell line is isolated from the peripheral blood of a 1-year old male patient suffering from acute monocytic leukemia [7]. This cell line has been widely used … rise up nutrition west reading paWeb3.1.2.1 Labeling effector cells and target cells. 1. Count THP-1 cells. If cell number allows, duplicate or triplicate wells are set up for each condition. 2. Count the target cells. Take at least twice the number of cells needed for the assay, because some cells will be lost throughout washes. In ADCP assays target cell number is usually the ... rise up oh men of god ldsWebPublished protocols using the promonocytic cell line THP-1 have tended to result in cells that closely resemble classically-activated macrophages, differentiated in IFNγ and LPS. … rise up o flame lyricsWeb4. Incubate the culture at 37°C incubator with 5% CO 2. Cultures should be monitored daily. 5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for … rise up oh men of god youtube